Effect of Euphorbia supina Extract on Antioxidant Activity

We investigated the effect of Euphorbia supina extract on the production of cellular reactive oxygen species (ROS) in order to evaluate its antioxidative activity. The H2O2-induced ROS generation was measured with dichlorofluorescein-diacetate (DCFH-DA) assay. The acetone+methylene chloride (A+M) and methanol (MeOH) extracts dose-dependently decreased ROS production induced by H2O2 compared to the extract-free control during the 120 min assay. The MeOH extract showed a higher inhibitory effect on the A+M at all concentrations tested. All tested fractions showed a decrease in ROS production with increase in concentration. Treatments with n-hexane and 85% aqueous methanol (MeOH) fractions (0.5 mg/mL concentrations) showed 82% and 97% inhibition rate, respectively, suggesting that the 85% aqueous MeOH fraction had ROS scavenging effects. These results indicate that the 85% aqueous MeOH fraction inhibited cellular oxidation and may contain valuable active compounds.